DNA Replication
Welcome to DNA Replication Course!
This course will guide participants through the fascinating process of DNA replication, a fundamental mechanism ensuring genetic continuity in all living cells. We will explore the experimental discoveries that confirmed the semiconservative nature of replication, the molecular machinery involved, and the step-by-step process of DNA synthesis in both prokaryotic and eukaryotic systems. Key topics include replication origins, polymerases, proofreading mechanisms, and the challenges of replicating chromosome ends. By the end of the course, participants will have a thorough understanding of how DNA faithfully replicates and the critical enzymes that drive this essential process.
Why take this course?
Learn step-by-step how DNA replicates, ensuring genetic continuity in all living organisms.
Explore the key enzymes and molecular mechanisms involved in replication.
Understand the differences between prokaryotic and eukaryotic DNA replication.
Discover the challenges of telomere replication and the role of telomerase in chromosome stability.
Essential for anyone studying cell biology, genetics, or molecular medicine.
Course Content
Importance of Genetic Continuity
Semiconservative Replication
DNA Synthesis Mechanisms
Semiconservative Replication
Conservative Replication
Dispersive Replication
The Meselson–Stahl Experiment
Isotopes in DNA Replication
Buoyant Density Centrifugation
Taylor–Woods–Hughes Experiment
Autoradiography and Replication Evidence
Origin of Replication (oriC)
Bidirectional Replication
Bidirectional Replication
DNA Polymerases I, II, III, IV, and V
The DNA Pol III Holoenzyme
Sliding DNA Clamp
DNA Helicase and Unwinding of the Helix
Single-Stranded Binding Proteins (SSBs)
DNA Gyrase and Topoisomerases
RNA Primers and Primase Activity
Continuous and Discontinuous DNA Synthesis
Okazaki Fragments and Lagging Strand
The Role of the Holoenzyme in Synthesis
The Donut-Shaped Sliding DNA Clamp
Error Rate in DNA Polymerase III
Exonuclease Activity in Proofreading
Differences Between Prokaryotic and Eukaryotic Replication
Multiple Replication Origins in Eukaryotes
Role of Origin Recognition Complex (ORC)
Cell-Cycle Kinases and Replication Control
Polymerase Switching Mechanism
Role of Telomeres in Stability and Replication
Telomerase Function and Structure (TERC & TERT)
Prevention of Chromosome Shortening
Who Should Enroll?
- Students in molecular biology, genetics, and medicine who want to master DNA replication.
- Researchers and professionals working in genomics, biotechnology, and bioinformatics.
- Science educators and teachers looking for a structured way to explain DNA replication.
- Anyone interested in the molecular basis of heredity and cell division.
Mohmed Abdalfttah
InstructorPhD Candidate
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Testimonials
A Huge Thank You to Mohmed! Taking the RNA-seq course with Mohmed was an incredible experience! His way of teaching made complex concepts easy to grasp, and the knowledge I gained has been truly transformative. Thanks to his guidance, I’m now on the verge of publishing a research paper in this field!
Ahmed
China
My experience with Dr. Mohmed was truly exceptional and unlike any other courses I’ve taken before. His dedication to ensuring that you genuinely understand the concepts, along with his smooth and engaging teaching style, makes learning effortless and enjoyable. You never feel overwhelmed—on the contrary, every session is an opportunity to explore, expand your knowledge, and be amazed by the rapid advancements in science. His approach encourages curiosity, pushing you to seek more and constantly evolve. I’m beyond grateful for the time I spent learning with him! ❤️
Refan
Saudi Arabia
Mohamed is an exceptional bioinformatics and genetic data analysis expert with a structured and intuitive teaching approach. His dedication to education, despite his own academic commitments, sets him apart as a passionate and supportive mentor. He simplifies complex concepts, fosters deep understanding, and creates an inspiring learning environment. His leadership, integrity, and commitment to knowledge-sharing make him an outstanding educator.