AbdalfttahAcademy

DNA Replication

Welcome to DNA Replication Course!

This course will guide participants through the fascinating process of DNA replication, a fundamental mechanism ensuring genetic continuity in all living cells. We will explore the experimental discoveries that confirmed the semiconservative nature of replication, the molecular machinery involved, and the step-by-step process of DNA synthesis in both prokaryotic and eukaryotic systems. Key topics include replication origins, polymerases, proofreading mechanisms, and the challenges of replicating chromosome ends. By the end of the course, participants will have a thorough understanding of how DNA faithfully replicates and the critical enzymes that drive this essential process.

Why take this course?

  • Learn step-by-step how DNA replicates, ensuring genetic continuity in all living organisms.

  • Explore the key enzymes and molecular mechanisms involved in replication.

  • Understand the differences between prokaryotic and eukaryotic DNA replication.

  • Discover the challenges of telomere replication and the role of telomerase in chromosome stability.

  • Essential for anyone studying cell biology, genetics, or molecular medicine.

Course Content

    • Importance of Genetic Continuity

    • Semiconservative Replication

    • DNA Synthesis Mechanisms

    • Semiconservative Replication

    • Conservative Replication

    • Dispersive Replication

    • The Meselson–Stahl Experiment

    • Isotopes in DNA Replication

    • Buoyant Density Centrifugation

    • Taylor–Woods–Hughes Experiment

    • Autoradiography and Replication Evidence

    • Origin of Replication (oriC)

    • Bidirectional Replication

    • Bidirectional Replication

    • DNA Polymerases I, II, III, IV, and V

    • The DNA Pol III Holoenzyme

    • Sliding DNA Clamp

    • DNA Helicase and Unwinding of the Helix

    • Single-Stranded Binding Proteins (SSBs)

    • DNA Gyrase and Topoisomerases

    • RNA Primers and Primase Activity

    • Continuous and Discontinuous DNA Synthesis

    • Okazaki Fragments and Lagging Strand

    • The Role of the Holoenzyme in Synthesis

    • The Donut-Shaped Sliding DNA Clamp

    • Error Rate in DNA Polymerase III

    • Exonuclease Activity in Proofreading

    • Differences Between Prokaryotic and Eukaryotic Replication

    • Multiple Replication Origins in Eukaryotes

    • Role of Origin Recognition Complex (ORC)

    • Cell-Cycle Kinases and Replication Control

    • Polymerase Switching Mechanism

    • Role of Telomeres in Stability and Replication

    • Telomerase Function and Structure (TERC & TERT)

    • Prevention of Chromosome Shortening

Who Should Enroll?

  • - Students in molecular biology, genetics, and medicine who want to master DNA replication.

  • - Researchers and professionals working in genomics, biotechnology, and bioinformatics.

  • - Science educators and teachers looking for a structured way to explain DNA replication.

  • - Anyone interested in the molecular basis of heredity and cell division.

Instuctor Mohmed Abdalfttah Picture

Mohmed Abdalfttah

Instructor

PhD Candidate

Faculty of Medicine, Autonomous University of Madrid
National Cancer Research Center (CNIO)
DNA Replication
  • 14 Videos
  • Online
  • 4.5 hours
  • Free

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Testimonials

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Ahmed testimonial

A Huge Thank You to Mohmed! Taking the RNA-seq course with Mohmed was an incredible experience! His way of teaching made complex concepts easy to grasp, and the knowledge I gained has been truly transformative. Thanks to his guidance, I’m now on the verge of publishing a research paper in this field!

Ahmed

China
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Refan testimonial

My experience with Dr. Mohmed was truly exceptional and unlike any other courses I’ve taken before. His dedication to ensuring that you genuinely understand the concepts, along with his smooth and engaging teaching style, makes learning effortless and enjoyable. You never feel overwhelmed—on the contrary, every session is an opportunity to explore, expand your knowledge, and be amazed by the rapid advancements in science. His approach encourages curiosity, pushing you to seek more and constantly evolve. I’m beyond grateful for the time I spent learning with him! ❤️

Refan

Saudi Arabia
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Rania testimonial

Mohamed is an exceptional bioinformatics and genetic data analysis expert with a structured and intuitive teaching approach. His dedication to education, despite his own academic commitments, sets him apart as a passionate and supportive mentor. He simplifies complex concepts, fosters deep understanding, and creates an inspiring learning environment. His leadership, integrity, and commitment to knowledge-sharing make him an outstanding educator.

Rania

USA

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