AbdalfttahAcademy

RNA Transcription

Welcome to RNA Transcription Course!

This course is designed to provide an in-depth exploration of transcription, the first step in gene expression where genetic information is transcribed from DNA into RNA. We will cover the molecular machinery that drives transcription, including RNA polymerases, promoters, and regulatory elements. Participants will learn about the genetic code, RNA processing events like splicing and polyadenylation, and the differences between prokaryotic and eukaryotic transcription. By the end of the course, participants will have a strong understanding of how transcription is regulated and its crucial role in cellular function and gene expression.

Why take this course?

  • Learn how genetic information is transcribed from DNA to RNA, a critical step in gene expression.

  • Understand the molecular machinery behind transcription, including RNA polymerase and transcription factors.

  • Explore the differences between prokaryotic and eukaryotic transcription.

  • Gain insights into post-transcriptional modifications, including splicing, polyadenylation, and RNA editing.

  • Essential for those interested in gene regulation, molecular genetics, and biotechnology applications.

Course Content

    • Genetic Information Encoding

    • DNA to RNA Information Transfer

    • Triplet Code and Codon Specificity

    • Start and Stop Codons

    • Nonoverlapping and Comma-less Nature of the Code

    • Colinearity and Universality

    • The Role of mRNA

    • Discovery of Messenger RNA (mRNA)

    • The Triplet Nature of the Genetic Code

    • Frameshift Mutations and Crick’s Experiment

    • Nirenberg and Matthaei’s Discoveries

    • Cell-Free Protein Synthesis Systems

    • Polynucleotide Phosphorylase and RNA Synthesis

    • Homopolymer Experiments

    • Triplet Binding Assay

    • Repeating Copolymer Experiments

    • Degeneracy and Codon Assignments

    • Wobble Hypothesis and tRNA Flexibility

    • Start Codon: AUG and Methionine

    • Stop Codons: UAA, UAG, UGA

    • Nonsense Mutations and Protein Truncation

    • Mitochondrial Code Variations

    • Evolutionary Adaptations of the Code

    • Role of RNA Polymerase

    • Promoters and Template Binding

    • Cis-Acting Elements: Promoters and Terminators

    • Consensus Sequences and Their Impact

    • Strength of Promoters and Gene Expression Levels

    • Sigma Factors and Their Role in Bacteria

    • Proofreading and RNA Polymerase Fidelity

    • Intrinsic and Rho-Dependent Termination

    • Nucleus vs. Cytoplasmic Transcription

    • Chromatin Remodeling in Eukaryotes

    • Cis-Acting Enhancers and Silencers

    • RNA Polymerase I, II, and III

    • Core Promoter and Enhancers

    • General and Specific Transcription Factors

    • Capping and Polyadenylation

    • Processing of mRNA in Eukaryotes

    • Alternative Splicing and Exon Shuffling

    • Self-Splicing Introns (Group I and II)

    • Spliceosome and Small Nuclear RNAs (snRNAs)

    • Role of snRNPs in Pre-mRNA Processing

    • Alternative Splicing and Gene Diversity

    • Evolutionary Importance of Introns

    • Regulatory Functions of Intronic Sequences

    • Cleavage and Polyadenylation

    • Regulation of mRNA Stability

Who Should Enroll?

  • - Students in biology, genetics, and biotechnology who want a deeper understanding of transcription.

  • - Researchers working in genomics, transcriptomics, and molecular biology.

  • - Science communicators and educators explaining gene expression and regulation.

  • - Science communicators and educators explaining gene expression and regulation.

Instuctor Mohmed Abdalfttah Picture

Mohmed Abdalfttah

Instructor

PhD Candidate

Faculty of Medicine, Autonomous University of Madrid
National Cancer Research Center (CNIO)
RNA Transcription
  • 19 Videos
  • Online
  • 7 hours
  • Free

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Testimonials

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Ahmed testimonial

A Huge Thank You to Mohmed! Taking the RNA-seq course with Mohmed was an incredible experience! His way of teaching made complex concepts easy to grasp, and the knowledge I gained has been truly transformative. Thanks to his guidance, I’m now on the verge of publishing a research paper in this field!

Ahmed

China
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Refan testimonial

My experience with Dr. Mohmed was truly exceptional and unlike any other courses I’ve taken before. His dedication to ensuring that you genuinely understand the concepts, along with his smooth and engaging teaching style, makes learning effortless and enjoyable. You never feel overwhelmed—on the contrary, every session is an opportunity to explore, expand your knowledge, and be amazed by the rapid advancements in science. His approach encourages curiosity, pushing you to seek more and constantly evolve. I’m beyond grateful for the time I spent learning with him! ❤️

Refan

Saudi Arabia
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Rania testimonial

Mohamed is an exceptional bioinformatics and genetic data analysis expert with a structured and intuitive teaching approach. His dedication to education, despite his own academic commitments, sets him apart as a passionate and supportive mentor. He simplifies complex concepts, fosters deep understanding, and creates an inspiring learning environment. His leadership, integrity, and commitment to knowledge-sharing make him an outstanding educator.

Rania

USA

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